Treatment with RO-82 (120 mg/kg) facilitates the recovery after HKBA-induced anemia. (A) Female (9-week-old) mice were treated with a single IP injection of HKBA (∼5 × 108 particles/mouse) at day 0. Then they were treated on day 8 and 11 (vertical arrows) with a dose of RO-82 heparin (120 mg/kg) or with saline, and the Hb level was monitored at days 7, 14, and 22. After the last bleeding, the mice were treated with another injection of RO-82 or saline, euthanized after 6 hours, and had their liver and serum analyzed. Transcripts of liver hepcidin, Id1, and Crp were evaluated by qRT-PCR, referred to Hprt1, and expressed as fold increase (B,E-F). The concentration of serum hepcidin (C) and serum iron (D) are also shown. At least 3 animals per group asterisks (*) indicate a significant difference (P < .05).
