A subset of pups born to multiparous wild-type females mated with KEL males are stillborn or pale. (A) Photograph of a representative pink KEL-negative and pale KEL-positive pup, hours after birth to a multiparous (third litter) wild-type female mated with a KEL male. (B) Representative KEL-specific PCR. (C) Flow cytometric analysis of the blood of the pups shown, with 1000 Trucount beads and RBCs gated. (D) TER119 positivity of gated RBCs. (E) Thiazole orange reticulocyte staining and (F) TER119 staining of the blood of the same pups. (G) Blood smear of representative KEL-negative and KEL-positive pups. (H) MRI of first (left) or third (right) pregnancies of representative wild-type females bred with KEL males.