Transfused KEL RBCs are selectively cleared in alloimmunized animals, with recipient proinflammatory cytokine response. (A) KEL and wild-type RBCs were labeled with DiI and DiO, respectively, prior to transfusion into females alloimmunized through pregnancy or into control recipients. (B) Post-transfusion survival and recovery of KEL RBCs was determined by comparing a ratio of DiI KEL to DiO wild-type RBCs. (C) RBC bound IgG was evaluated by flow cytometry 10 minutes post-transfusion. (D-F) Serum cytokine responses in alloimmunized animals 90 to 120 minutes after a KEL RBC transfusion. Results are representative (A-C) or a compilation (D-F) of 3 experiments (n = 18 animals). *P < .05.