CD107a-expressing target cells are less sensitive to Pfn-mediated apoptosis induction. (A) HeLa cells were coincubated with sonicated CGs purified from YTS cells. After 4 hours, cells were stained with Annexin-V to assess apoptotic cells. Top: Annexin-V staining of the indicated cells with or without granules. Bottom: Median Annexin-V fluorescence intensities at different granule dilutions. (B) Relative median fluorescence of 3 independent experiments as depicted in (A). Statistical analysis was performed using 2-way ANOVA; n = 6. (C) Representative surface staining of Pfn on the indicated HeLa cells after 4 hours of coincubation with sonicated CGs; n = 8.