Tissue cellularity, progenitor and early cell content in KI (gray bars), and WT littermate (white bars) mice. (A) Spleen weight (also showing spleens collected in 6- and 12-week-old KI mice) and BM cell content (6-12 weeks) per femur in KI (n = 11) and WT littermate (n = 9) mice. (B) Total numbers (×103) in BM (top), spleen (SP, middle) and cumulative numbers (×103) in BM plus spleen (BM+SP) of CFU-GM, BFU-E and CFU-E from KI (n = 9) and WT littermate (n = 7) mice. CFU-E numbers from KI mice growing without added Epo (endogenous colonies) are represented in the black bars (NS for nonstimulated). No endogenous CFU-E was found in organs from WT littermate mice. The calculation is detailed in “Methods.” (C) Percentages of Lin-, LSK, and SLAM cells in BM and spleen from KI and WT littermates measured by FACS at 1, 3, and 6 months of age, n = 3 mice for 1- and 6-month-old mice and n = 8-12 mice for 3-month-old mice. (D) Total numbers (×106) of Lin-, LSK, and SLAM (LSK, CD48−, CD150+) cells in BM plus spleen from KI and WT littermates measured at 1, 3, and 6 months of age. Calculation is detailed in “Methods” from percentage of Lin-, LSK, and SLAM cells represented in C. n = 3 for SLAM, LSK, and Lin- in 6-month-old mice or 8-9 mice for 3-month-old mice. Results are mean value ± SEM. *P ≤ .05 with the 2-tailed unpaired Student t test.
