CX3CR1 and CCR2 have opposing actions in monocyte recovery after myeloablation. Representative dot plots previously gated on CD11b⁺ cells indicate the gating strategy for a Ly6C^high monocyte subset from the bone marrow (A), blood (B), and spleen (C) (percent ± standard deviation [SD] of the gated subset from untreated mice are indicated). Kinetic of Ly6C^high monocyte recovery after CP treatment (175 mg/kg) in Balb/c (empty), CCR2^−/− (gray), and CX3CR1^−/− (black) mice was monitored. D0 corresponds to untreated mice. Graph represents the absolute number ± standard error of the mean (SEM) of Ly6C^high monocytes after CP treatment in bone marrow, blood, and spleen (n = 6-15 mice for each time point out of at least 3 independent experiments). A 2-way analysis of variance (ANOVA) with Bonferroni comparison post-test was performed. Statistical significance between WT and knockout strains is indicated.