CX3CR1 reduced medullar Ly6C^high monocyte release into the bloodstream. (A) Representative dot plots previously gated on CD11b⁺ cells indicate the gating strategy in the bone marrow, blood, and spleen for Ly6C^high- and Ly6C^low-myeloid subsets based on CX3CR1 expression using CX3CR1^gfp/+ mice. (B) Bars represent absolute number ± SEM of the 2 monocyte subsets before and 5 days after CP treatment in bone marrow, blood, and spleen of CX3CR1^gfp/+ and CX3CR1^gfp/gfp mice (n = at least 12 mice for each time point pooled from 5 independent experiments; a 1-way ANOVA with Bonferroni comparison post-test was performed). (C) Bone marrow cells from CX3CR1^gfp/+ and CX3CR1^gfp/gfp mice were harvested 5 days after CP treatment, labeled with CMTMR and Hoechst, respectively, and grafted into C57Bl6 recipient mice 2 days after CP treatment. The day after, bone marrow, blood, and spleen were harvested and the percent of transferred cells recovered was calculated for each organ and per mL of blood. Dot plots indicate the gating strategy of transferred cell recovery. Ctrl, control; D, day; NT, not treated; SSC, saline sodium citrate.