CP treatment reduced the CX3CR1-dependent adherence of marrow monocytes. (A) Dot plots represent Ly6C and GFP expression gated on CD11b⁺Ly6G⁻ cells on both bone marrow monocyte subsets at different time points after CP treatment of CX3CR1^gfp/+ mice. Mean GFP MFI ± SD is indicated (n = 5-10 mice per time point out of 3 independent experiments; a 1-way ANOVA with Bonferroni comparison post-test was performed). (B) Representative histogram plot overlay of CX3CL1–Alexa 647 expression gated on bone marrow GFP⁺ cells in each indicated conditions. GFP^high-expressing cells corresponding to Ly6C^low monocytes have been excluded from the gating. Graph represents mean ± SEM of the CX3CL1–Alexa 647 MFI in each condition (n = 5-9 different mice out of 4 independent experiments; 1-way ANOVA with Bonferroni comparison post-test was performed). (C) Graph represents mean ± SEM of the percentage of adherent GFP⁺ bone marrow cells on CX3CL1-coated wells from CP-treated or untreated CX3CR1^gfp/+ and CX3CR1^gfp/gfp mice. To evaluate adherence specificity to coated CX3CL1, cells have been previously incubated or not with soluble CX3CL1. GFP⁺ adherent cells on uncoated wells have been subtracted for each condition. Data are collected from triplicate out of 2 independent experiments; a 1-way ANOVA with Bonferroni comparison post-test was performed. D, day; KO, knockout; ND, not determined; NT, not treated.