CP treatment induced monocytes redistribution in the bone marrow. TPLSM images of monocyte cells (cyan) in parenchymal zones (A) and bone marrow vasculature (B) of skull bone tissue of MacBlue mice. The vasculature is in red (rhodamine-dextran) and the bone matrix is in blue (SHG). White bars represent 50 µm. Dot plots (upper right corner) indicate the percentage of Ly6-C^high monocytes among CFP⁺ cells. Graphs represent the distribution of track straightness as a function of cell mean velocity after CP treatment. Quadrants separate cells according to track straightness (value is 0.25 for parenchyma and 0.5 for vasculature) and mean velocity (2 μm/min for parenchyma and 4 μm/min for vasculature) (corresponding to the median values for each compartments in untreated mice). Quadrant statistics are indicated for each condition (parenchyma: not treated [NT] n = 687, day [D] 1 n = 327, D3 n = 219, D5 n = 448; vasculature: NT n = 583, D1 n = 899, D3 n = 566, D5 n = 498; from at least 3 different mice in each condition).