Figure 1
Figure 1. PRCP levels modulate endothelial cell growth. (A) BAEC were transfected with control or PRCP siRNA, and images were taken at time 0 (D0) and at 24 and 48 hours. (B) Graphic data represent mean ± SD changes in cell numbers per field compared with mean D0 values (n = 3 for both groups). (C) Relative bovine PRCP mRNA expression after siRNA transfections (Cont vs PRCP) on quantitative PCR (n = 5 for both groups). (D) Relative PK activation by expressed bovine PRCP as determined by OD405 nm/mg protein of cells (n = 3 for both groups). (E) BAEC were transfected with Control (vector) or hPRCP (plasmid expressing the full length mature human PRCP), and images were taken at time 0 (D0) and at 24 and 48 hours. (F) Graphic data represent mean ± SD changes in cell numbers per field compared with mean D0 values (n = 3 for both groups). (G) Relative human PRCP mRNA expression after vector (Cont) or hPRCP transfection on quantitative PCR (n = 5 for both groups). (H) Relative PK activation by expressed PRCP after hPRCP transfection as determined by OD405 nm/mg protein of cells (n = 9 in both groups). (I) The % growth change of BAEC after siRNA (Cont or PRCP) or plasmid (Cont, hPRCP, hPRCPmut) transfections as measured by a MTS cell assay (n ≥ 5 for all groups). (J) The % growth change of BAEC transfected with PRCP siRNA (+) (n = 9) without (C) or with hPRCP (n = 15) or hPRCPmut plasmid (n = 15). (K) Alexa-594–labeled Annexin V expression on BAEC after Control and PRCP siRNA transfection. (L) The relative Annexin V expression (pixels/field) on BAEC after Control and PRCP siRNA transfection from (K) (n ≥ 12 for both groups). (M) Alexa-594–labeled Annexin V expression on BAEC after Control and hPRCP transfection. (N) The relative pixels/field of Annexin V expression on BAEC after Control and hPRCP transfection from (M) (n > 12 for both groups). All images were obtained using a Nikon TE200 microscope with a 10×/0.25 objective lens. The figures are expressed as mean ± SEM unless otherwise stated. *P < .05, **P < .01, ***P < .001 of comparisons between 2 groups.

PRCP levels modulate endothelial cell growth. (A) BAEC were transfected with control or PRCP siRNA, and images were taken at time 0 (D0) and at 24 and 48 hours. (B) Graphic data represent mean ± SD changes in cell numbers per field compared with mean D0 values (n = 3 for both groups). (C) Relative bovine PRCP mRNA expression after siRNA transfections (Cont vs PRCP) on quantitative PCR (n = 5 for both groups). (D) Relative PK activation by expressed bovine PRCP as determined by OD405 nm/mg protein of cells (n = 3 for both groups). (E) BAEC were transfected with Control (vector) or hPRCP (plasmid expressing the full length mature human PRCP), and images were taken at time 0 (D0) and at 24 and 48 hours. (F) Graphic data represent mean ± SD changes in cell numbers per field compared with mean D0 values (n = 3 for both groups). (G) Relative human PRCP mRNA expression after vector (Cont) or hPRCP transfection on quantitative PCR (n = 5 for both groups). (H) Relative PK activation by expressed PRCP after hPRCP transfection as determined by OD405 nm/mg protein of cells (n = 9 in both groups). (I) The % growth change of BAEC after siRNA (Cont or PRCP) or plasmid (Cont, hPRCP, hPRCPmut) transfections as measured by a MTS cell assay (n ≥ 5 for all groups). (J) The % growth change of BAEC transfected with PRCP siRNA (+) (n = 9) without (C) or with hPRCP (n = 15) or hPRCPmut plasmid (n = 15). (K) Alexa-594–labeled Annexin V expression on BAEC after Control and PRCP siRNA transfection. (L) The relative Annexin V expression (pixels/field) on BAEC after Control and PRCP siRNA transfection from (K) (n ≥ 12 for both groups). (M) Alexa-594–labeled Annexin V expression on BAEC after Control and hPRCP transfection. (N) The relative pixels/field of Annexin V expression on BAEC after Control and hPRCP transfection from (M) (n > 12 for both groups). All images were obtained using a Nikon TE200 microscope with a 10×/0.25 objective lens. The figures are expressed as mean ± SEM unless otherwise stated. *P < .05, **P < .01, ***P < .001 of comparisons between 2 groups.

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