PRCP influences limb ischemia recovery. (A) LDI was performed on hind limbs and feet from WT and PRCPgt/gt mice (n = 10 for both groups) before and after ligation of the femoral artery on D0, D7, D14, and D21. The figure is a representative study. The scale of color from red to blue on the right represents the intensity of heat from strong to little. (B) LDI densities of the blood flow in injured limbs were compared with the uninjured limbs for each genotype on each day. On 2-way analysis of variance, the differences between the 2 recovery curves were highly significant (P < .0001). (C) Frozen sections from gastrocnemius muscles were stained with a PECAM (CD31) antibody from uninjured and ischemic limbs after the ligated vessel was excised and removed (n = 4 for both groups) 28 days after injury. Images were obtained using a Nikon TE200 microscope 20×/0.45. (D) CD31-positive vessel area in pixels was determined by morphometric analysis in each of the control or treatment groups. *P < .05, **P < .01 for specific comparison between groups.
