American Society of Hematology

Figure 1

$Figure 1. TTT-3002 is a potent inhibitor of FLT3/ITD in AML cell lines. (A) Structure of TTT-3002. (B) Inhibition of pFLT3 in Molm14 and MV4-11 cells treated with TTT-3002 or AC220; fraction of pFLT3/FLT3 relative to DMSO control is indicated below each western blot. (C) Viable cell counts by Trypan blue exclusion assay; error bars represent average ± standard deviation (SD). (D) Inhibition of colony formation by TTT-3002; error bars represent average ± SD. (E) Cell cycle arrest at 24 hours following treatment with TTT-3002 (0 to 10 nM); error bars represent average ± SD. (F) Annexin V binding at 48 hours; data represent average percentage of Annexin V–positive cells ± SD. (G) Expression of proapoptotic markers, cleaved poly ADP ribose polymerase (PARP), and cleaved caspase-3 was increased at 12 and 24 hours by western blotting of Molm14 and MV4-11 cell lysates following treatment with TTT-3002 at the indicated concentrations.$

TTT-3002 is a potent inhibitor of FLT3/ITD in AML cell lines. (A) Structure of TTT-3002. (B) Inhibition of pFLT3 in Molm14 and MV4-11 cells treated with TTT-3002 or AC220; fraction of pFLT3/FLT3 relative to DMSO control is indicated below each western blot. (C) Viable cell counts by Trypan blue exclusion assay; error bars represent average ± standard deviation (SD). (D) Inhibition of colony formation by TTT-3002; error bars represent average ± SD. (E) Cell cycle arrest at 24 hours following treatment with TTT-3002 (0 to 10 nM); error bars represent average ± SD. (F) Annexin V binding at 48 hours; data represent average percentage of Annexin V–positive cells ± SD. (G) Expression of proapoptotic markers, cleaved poly ADP ribose polymerase (PARP), and cleaved caspase-3 was increased at 12 and 24 hours by western blotting of Molm14 and MV4-11 cell lysates following treatment with TTT-3002 at the indicated concentrations.

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