Inhibition of PLC downstream signaling affects cell proliferation, survival, and NFAT activity in CTCL cells. Cell proliferation assay in Myla (A) and HUT78 (B) cells incubated for 0, 24, or 48 hours, using DMSO (control) or the indicated amount of FK-506 (µM). N = 3, error bars indicate SEM. Percentage of viable Myla (C) and HUT78 (D) cells incubated for 24 hours with vehicle (DMSO) or the indicated fold 50% inhibitory dose concentration of FK-506 (50% inhibitory dose values at 48 hours for Myla and HUT78 were 27.4 and 18.9 µM, respectively). Right plots are representative examples for AnnexinV (X-axis)/7AAD (Y-axis) staining data in each case. Representative western blot using anti–poly ADP ribose polymerase and anti-tubulin in whole protein Myla (E) and HUT78 (F) cell lysates treated with DMSO or FK-506 as in C and D. Percentage of NFAT activity in nuclear extracts from Myla (G) or HUT78 (H) cells treated with DMSO (vehicle) or FK-506 in the same conditions as above (Figure 3C-F). N = 3; error bars indicate SEM.