Evaluation of lectin extravasation with optical projection tomography. Right-sided growth factor injections were performed accompanied by left-sided sham operation. After VEGF gene transfer, major extravasation can be observed at the site of the hemangioma (arrowhead: A,C,E). PlGF gene transfer induced the formation of a dense microcapillary network, without significant extravasation (arrow: B,D,F). Autofluorescence is shown in green; lectin staining of blood vessels and extravasation in blue. ca, caudal; Ro, rostral.