IVIg induces COX-2 and PGE₂ in DCs. (A) DCs were cultured in GM-CSF and IL-4 alone (Ctr) or with IVIg (10 mg or 15 mg) or HSA for 24 hours. Expression of COX-2 in DCs was analyzed by western blot. Representative blot of 7 independent experiments is shown. (B) The fold changes in COX-2 expression based on densitometry analysis of western blots (mean ± SEM, n = 7). AU, arbitrary units. (C) The level (mean ± SEM, n = 7) of production of PGE₂ (pg/mL) by DCs under the experimental conditions as explained above, * P < .05; ***P < .001.