IL-10 is required for Treg-mediated disruption of Tcon–DC interaction. Intravital imaging of donor T cells (labeled with CMTPX) and host DCs in the popliteal LNs of irradiated CD11c-DTR-EGFP mice in the presence of WT or IL-10−/− Tregs. (A) The comparison of mean velocity of donor T cells in the presence IL-10−/− or WT Tregs. (B) The comparison of the displacement ratio of donor T-cell movement in the presence IL-10−/− or WT Tregs. (C) Contact time between donor T cells and host DCs in the presence IL-10−/− or WT Tregs at different time points posttransplant. The bars in all graphs represent median values. Asterisks indicate statistically significant differences between the groups or time points (*P < .05). Data are representative of 2 experiments with 1 to 2 mice per experiment.
