American Society of Hematology

Figure 1

$Figure 1. Repositioning of IgH and Ccnd1 in MCL cells. FISH was performed on 3-dimensionally preserved Granta-519 cell nuclei to reveal the IgH and Ccnd1 alleles. (A) Schematic representation of the chromosomes involved in the t(11;14) translocation in MCL cells and localization of the IgH and Ccnd1 probes used. (B) Distribution of the translocated (der14) and nontranslocated IgH and Ccnd1 alleles according to their radial positioning in Granta-519 cell nuclei. Distances were measured as described in Materials and methods. Histograms represent the percentage of FISH signals in the nuclear space with the range 0.0 to 0.15 corresponding to the centermost fraction of the nucleus and 0.75 to 1 to its periphery. Each graph represents a minimum of 120 FISH signals.$

Repositioning of IgH and Ccnd1 in MCL cells. FISH was performed on 3-dimensionally preserved Granta-519 cell nuclei to reveal the IgH and Ccnd1 alleles. (A) Schematic representation of the chromosomes involved in the t(11;14) translocation in MCL cells and localization of the IgH and Ccnd1 probes used. (B) Distribution of the translocated (der14) and nontranslocated IgH and Ccnd1 alleles according to their radial positioning in Granta-519 cell nuclei. Distances were measured as described in Materials and methods. Histograms represent the percentage of FISH signals in the nuclear space with the range 0.0 to 0.15 corresponding to the centermost fraction of the nucleus and 0.75 to 1 to its periphery. Each graph represents a minimum of 120 FISH signals.

This Feature Is Available To Subscribers Only