Perinucleolar relocalization of the translocated Ccnd1 allele in MCL patients. (A) Graphs show the distance of FISH signals to the nucleoli. Each graph corresponds to one patient with MCL. Distances have been measured in at least 100 nuclei. ***P < .001. (B) Representative images of double-labeled DNA FISH for each patient. IgH is green, Ccnd1 is red, and nucleolus (B23) is blue. Immuno-FISH and distance measurements were performed as in Figure 2, specifically on cells with the t(11;14) translocation, which were detected by colocalization of the IgH and Ccnd1 signals in the nuclei. Scale bar represents 5 μm.