Simultaneous detection of fluorescently labeled transcription factories, nucleoli, heterochromatin, IgH, and Ccnd1 alleles in the Granta-519 MCL cell line. (A) (i) Active RNA PolII (green) and nucleoli (B23, blue) were immunostained with the nucleus counterstained with 4,6 diamidino-2-phenylindole (gray); (ii) enlargement focused on the nucleolus. Scale bar represents 3 μm. (B) IgH (green, i), Ccnd1 (red, ii), active RNA PolII (gray, iii), and nucleoli (B23, blue, iv) were simultaneously revealed by immuno-FISH. In merged images shown in (v) (merged from i, ii, and iv) and (vi) (merged from i, ii, iii, and iv), the arrow points to the der14-carried IgH and Ccnd1 alleles. Scale bar represents 3 µm.