Figure 5
Figure 5. Anaphylaxis caused by DTA-1 is mediated by PAF, basophils, and IgG1 antibodies. (A) C57BL/6J or Kitw/KitW-v mice were treated with 1 mg DTA-1 on days 0, 4, and 11. Rectal temperatures of individual mice were monitored for 1 hour following the final injection, represented by individual lines in the graphs. Data are representative of 2 independent experiments (n = 5 mice per group). (B) C57BL/6J mice were treated with 1 mg DTA-1 on days 0, 4, and 11. Thirty minutes prior to the final DTA-1 injection, mice were injected intraperitoneally with either 125 µg of the histamine inhibitor triprolidine or 125 µg of the PAF inhibitor CV6209. Rectal temperatures of individual mice were monitored for 1 hour following the final dose of DTA-1, represented by individual lines in the graphs. Data are representative of 3 independent experiments (n = 5 mice per group). (C) C57BL/6J mice were treated with 1 mg DTA-1 on days 0, 4, and 11. One day before the final dose of DTA-1, mice were either injected intravenously with 25 µg of anti-CD200R3 (clone Ba103) or intraperitoneally with 0.5 mg of anti-Ly6G (clone 1A8). Rectal temperatures of individual mice were monitored for 1 hour following the final dose of DTA-1, represented by individual lines in the graphs. Data are representative of 3 independent experiments (n = 5 mice per group). (D) Sera from DTA-1–treated mice were collected as in Figure 4A. The pooled sera were heat inactivated at 56°C for 3 hours. Heat-inactivated sera was then transferred by intravenous injection into the tail vein of recipient mice. Three hours later, a single dose of DTA-1 was administered, and rectal temperatures were monitored, represented by individual lines in the graphs. Data are representative of 2 independent experiments (n = 5 mice per group). (E) C57BL/6J mice were treated with DTA-1 or isotype control (rat IgG2b, clone LTF-2) on days 0 and 4. On day 11, sera were collected from individual mice and assayed by enzyme-linked immunosorbent assay for IgG1 antibodies recognizing DTA-1 or LTF-2. Data are representative of 3 independent experiments.

Anaphylaxis caused by DTA-1 is mediated by PAF, basophils, and IgG1 antibodies. (A) C57BL/6J or Kitw/KitW-v mice were treated with 1 mg DTA-1 on days 0, 4, and 11. Rectal temperatures of individual mice were monitored for 1 hour following the final injection, represented by individual lines in the graphs. Data are representative of 2 independent experiments (n = 5 mice per group). (B) C57BL/6J mice were treated with 1 mg DTA-1 on days 0, 4, and 11. Thirty minutes prior to the final DTA-1 injection, mice were injected intraperitoneally with either 125 µg of the histamine inhibitor triprolidine or 125 µg of the PAF inhibitor CV6209. Rectal temperatures of individual mice were monitored for 1 hour following the final dose of DTA-1, represented by individual lines in the graphs. Data are representative of 3 independent experiments (n = 5 mice per group). (C) C57BL/6J mice were treated with 1 mg DTA-1 on days 0, 4, and 11. One day before the final dose of DTA-1, mice were either injected intravenously with 25 µg of anti-CD200R3 (clone Ba103) or intraperitoneally with 0.5 mg of anti-Ly6G (clone 1A8). Rectal temperatures of individual mice were monitored for 1 hour following the final dose of DTA-1, represented by individual lines in the graphs. Data are representative of 3 independent experiments (n = 5 mice per group). (D) Sera from DTA-1–treated mice were collected as in Figure 4A. The pooled sera were heat inactivated at 56°C for 3 hours. Heat-inactivated sera was then transferred by intravenous injection into the tail vein of recipient mice. Three hours later, a single dose of DTA-1 was administered, and rectal temperatures were monitored, represented by individual lines in the graphs. Data are representative of 2 independent experiments (n = 5 mice per group). (E) C57BL/6J mice were treated with DTA-1 or isotype control (rat IgG2b, clone LTF-2) on days 0 and 4. On day 11, sera were collected from individual mice and assayed by enzyme-linked immunosorbent assay for IgG1 antibodies recognizing DTA-1 or LTF-2. Data are representative of 3 independent experiments.

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