Effect of JMJD1C depletion on human leukemic cells. (A) Relative JMJD1C mRNA levels in a panel of human leukemic cell lines normalized to mRNA levels in SEM cells. (B) Box plots of mean JMJD1C mRNA levels in MLL-rearranged and non–MLL-rearranged cell lines from (A). (C) Relative cell number of the indicated cell lines transduced with shScr, shJMJD1C#1, or shJMJD1C#2 at day 8 after GFP+ sort. Cells were sorted by FACS 4 days after transduction. Error bars indicate standard deviation (n = 3 technical replicates). See also supplemental Figure 2. (D) Number of colonies generated in semisolid media by GFP+ cells transduced with shScr, shJMJD1C#1, or shJMJD1C#2. Error bars indicate standard deviation (n = 3 technical replicates).