LIN28B overexpression in adult human erythroblasts.LIN28B-OE was confirmed by (A) qRT-PCR quantitation of copy number per nanogram complementary DNA (cDNA) (copies/ng cDNA); (B) Western blot analysis and (C-F) confocal images of control and LIN28B-OE cells were stained with DAPI (4′,6-diamidino-2-phenylindole) (blue) and LIN28B (green). Cells were transfected with empty vector (C-D), and LIN28B (E-F). Analyses were performed at culture day 14. Mean value ± standard deviation of 3 independent donors for each condition. C, empty vector control; OE, LIN28B overexpression.