LIN28B overexpression enhances HbF levels in adult human erythroblasts. Flow cytometry analyses of (A) control and (B) LIN28B-OE at culture day 14, and (C) control, and (D) LIN28B-OE at culture day 21 stained with anti-transferrin receptor (CD71) and anti-glycophorin A (GPA) antibodies. Enucleation is represented by staining of culture day 21 cells with thiazole orange for (E) control and (F) LIN28B-OE. The enucleated cells were imaged in panels (G) control and (H) LIN28B-OE. HPLC analysis of hemoglobin from (I) control and (J) LIN28B-OE, and (K) control and (L) LIN28A-OE samples performed at culture day 21 shown for comparison with LIN28B-OE. HbF and HbA peaks are labeled on each graph (y-axis: mVolts; x-axis: elution time in minutes). Data are representative of more than 3 independent experiments. LIN28B-OE, LIN28B overexpression; LIN28A-OE, LIN28A overexpression.