LIN28B expression regulates γ-globin and erythroid-related fetal genes in adult erythroblasts. (A) α, μ, θ, and ζ globins, (B) β, δ, γ, and ε globins, (C) CA1, and (D) GCNT2 mRNA expression analysis of LIN28B-OE compared with control samples. Open bars represent control and black bars represent LIN28B-OE. qRT-PCR analyses were performed at culture day 14. Mean value ± standard deviation of 3 independent donors for each condition. P values were calculated using two-tailed Student t test. C, empty vector control; OE, LIN28B overexpression. *P < .05.