LIN28B modulation of adult CD34+ cells toward a fetal-like phenotype involves the let-7 family of miRNAs, miR-96, and BCL11A.LIN28B-OE compared with control samples in (A) the relative expression levels of the let-7 family of miRNAs (open bars represent control and black bars represent LIN28B-OE), the mRNA expression levels of (B) HMGA2 and (C) IGF2, (D) the relative expression levels of miR-96, miR-29c, miR-451, miR-144, and miR-142, and the mRNA expression of the transcription factors (E) BCL11A, (F) GATA1, (G) KLF1, and (H) SOX6. The qRT-PCR analyses were performed at culture day 14. The miRNAs relative expression levels (y-axis) in the control cells were defined as a level of one for comparison. Error bars denote ± standard deviation of 3 independent donors for each condition. P values were calculated using two-tailed Student t test. C, empty vector control; OE, LIN28B overexpression. *P < .05.