The CNTRL-FGFR1 fusion kinase induces BaF3 cell transformation. (A) Scheme for construction of the MIEG3-CNTRL-FGFR1. (B) Flow cytometry analysis shows that almost all BaF3 cells transformed by CNTRL-FGFR1 are viable and express GFP after withdrawal of IL-3. (C) Cell proliferation assays demonstrate increased IL-3–independent growth of CNTRL-FGFR1-BaF3 cells. (D) Western blot analysis shows the presence of the CNTRL-FGFR1 fusion protein in stably transformed BaF3 cells.