Figure 3
Figure 3. hTERT genomic locus and hTERT expression. (A-C) hTERT status. (A-B) FISH investigation used bacterial artificial chromosome (BAC) probes specific for hTERT labeled with SpectrumRed, 5p13.1 genomic region labeled with SpectrumGreen, and 5q31.2 genomic region labeled with SpectrumGold. (A) hTERT diploid nucleus and metaphase. (B) Metaphase with 3 copies of chromosome 5 and an extra signal for the hTERT locus. Images were acquired by using Isis software (MetaSystems); objective ×63. (C) Array comparative genomic hybridization (aCGH) profile shows that a partial gain of chromosome 5 included the hTERT locus. (D) Real-time qRT-PCR assay to evaluate hTERT mRNA level. Compared with healthy lymphocytes, SS cells showed a slight increase of hTERT transcripts, but this was not statistically significant (P = .29).

hTERT genomic locus and hTERT expression. (A-C) hTERT status. (A-B) FISH investigation used bacterial artificial chromosome (BAC) probes specific for hTERT labeled with SpectrumRed, 5p13.1 genomic region labeled with SpectrumGreen, and 5q31.2 genomic region labeled with SpectrumGold. (A) hTERT diploid nucleus and metaphase. (B) Metaphase with 3 copies of chromosome 5 and an extra signal for the hTERT locus. Images were acquired by using Isis software (MetaSystems); objective ×63. (C) Array comparative genomic hybridization (aCGH) profile shows that a partial gain of chromosome 5 included the hTERT locus. (D) Real-time qRT-PCR assay to evaluate hTERT mRNA level. Compared with healthy lymphocytes, SS cells showed a slight increase of hTERT transcripts, but this was not statistically significant (P = .29).

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal