Transcription factor binding sites and histone modifications on the Major Peak. The binding profiles of Runx1, TCF-1, Ikaros, PU.1, and GATA-3 at the Major Peak region in T cells, measured by ChIP-seq, are shown.^9,24,25,37  Also shown are the alignments of the full MP sequence and the 528-bp region between the two TCF-1 binding peaks, which was deleted from Major Peak to make the MPFusion construct. Dark blue stars mark the two central TCF-1 sites that were mutated in the dmTCF construct. The 3′ conserved region is also indicated. For coordinates, see supplemental Table 2.