Stim1^−/−chimeras have less liver damage following ischemia/reperfusion injury. (A) WT or stim1^−/− chimeric mice were subjected to liver ischemia/reperfusion injury by ligation of the portal vein and hepatic artery for 30 minutes followed by 3 hours of reperfusion. Mice were then euthanized, and liver samples were obtained for histology. Liver sections were stained with trichrome (upper 4 panels) or H&E (lower 2 panels). Areas of poor trichome staining (arrowheads) indicate hepatic necrosis in the WT mice, which were absent in livers from stim1^−/− chimeras. Arrowheads indicate inflammatory cell infiltrate. Magnification is ×10 (upper panels), ×20 (middle panels) and ×40 (lower panels). Images shown are representative of 10 mice per group subjected to ischemia/reperfusion injury in separate experiments. (B) WT, stim1^−/− chimeras, or p47^phox-deficient mice were subjected to liver ischemia/reperfusion injury, and blood samples were obtained 3 hours after reperfusion. Liver enzymes aspartate aminotransferase and alanine aminotransferase were determined by a veterinary clinical chemistry analyzer. (C) The serum samples from above were analyzed for IL-6 by enzyme-linked immunosorbent assay. Data are averaged from 3 mice per group and are representative of 2 independent experiments. ***P < .001.