Thrombin generation in the FV_Nara patient’s plasma. (A) Effects of the addition of APC: thrombin generation after extrinsic activation (TF; 5 pM) of PPP (a) or PRP (b) in normal individuals (circle symbols) and PPP (a) or PRP (b) in FV_Nara patient (square symbols) in the absence (open symbols) or presence (closed symbols) of APC was measured as described in “Materials and methods.” With PPP, APC (8 nM) was added with PL vesicles (10 μM), whereas with PRP, APC (40 nM) was added without PL. A representative thrombogram is shown. (B) Effects of the addition of native FV: thrombin generation was measured after extrinsic activation (TF; 5 pM) of the patient’s PRP with the addition of native FV (circles, 0 IU/dL; squares, 10 IU/dL) in the absence (open symbols) or presence (closed symbols) of APC (40 nM). All experiments were performed at least 3 separate times, and a representative thrombogram is shown.