hGH promotes the differentiation of megakaryocytes. (A) Human cord blood-derived CD34⁺ cells were cultured in the presence of rhSCF (20 ng/ml) with or without different concentrations of rhGH (10, 50, and 100 ng/ml) or rhTPO (10 ng/ml) for 13 days. (i) The expressions of CD41 and CD42b in the cells treated with 100 ng/ml rhGH for 7, 10, and 13 days were analyzed by flow cytometry. (ii) Histogram showing the proportions of CD41⁺CD42b⁺ cells for each group. Data are from 3 independent experiments. Error bars denote SD. **P < .01. (iii) The influence of anti-TPOR treatment on the expression of CD41/42b induced by rhGH and rhTPO. Data are from 3 independent experiments. Error bars denote SD. **P < .01. (B) The increased numbers of cells in the culture at day 7 were analyzed by a cytometer and compared with seeding numbers of CD34⁺ cells at day 0. Data are from 3 independent experiments. Error bars denote SD. (C) Viability of M07e cells cultured with the indicated concentrations of rhGH for 72 hours as analyzed by CCK-8 assay. The data are from 6 independent assays with a single batch of cells. Error bars denote SD. **P < .01.