Platelet clearance in spleen and liver is reduced after a high-ALA diet. Platelet half-life was increased in the high-ALA compared with the low-ALA group (A; n = 6; P = .07). Liver cryosections were stained for the platelet marker CD41 (red) and the macrophage marker CD68 (green); images were taken with an Olympus microscope (B; magnification 20×; scale bar 100 μm). Colocalization is shown by a merge of the 2 fluorescent signals (yellow, arrows). Spleen and liver cryosections were also analyzed for the platelet marker CD41 (green); the positive area quantified with the software AnalySIS 3.1 (Soft Image Solutions) and was found to be significantly reduced in the high-ALA mice in both organs (C-D; n = 3; *P = .03; **P = .005). Macrophage number in the spleen and liver was not affected by the ALA treatment as shown by quantification of the CD68-positive area (E; n = 3; P > .05).