Mouse lncRNAs selected for RNAi studies. Twenty-seven lncRNAs were selected according to criteria described in the text. The relative expression of these lncRNAs in purified hematopoietic lineages from adult mouse bone marrow was determined using a custom oligonucleotide array and is shown graphically. “Conserved” refers to the presence of assembled orthologous transcripts detected in human erythroblasts (Figure 3C). Candidate erythroid lncRNAs shown here are upregulated 2- to 2054-fold (median 41-fold) in erythroblasts compared with multipotent progenitors. Names assigned to lncRNAs that potentially function in erythropoiesis according to RNAi studies in Figure 6 are indicated. Lnc051 was previously named “Lincred1.”32 Ggnbp2os was not detectable in primary human erythroblasts, but transcription cannot be ruled out in human K562 erythroleukemia cells (supplemental Figure 8). CMP, common myeloid progenitor; EE, early erythroblast; Expr, absolute expression level by microarray; GMP, granulocyte macrophage progenitor; GR, granulocyte; HSC, hematopoietic stem cell; LE, late erythroblast.
