Oxidative stress-associated immune alterations in CLL patients. (A) The MFI of CD69, HLA-DR, CD137, CD3ζ-chain, and the maturation status of CD4⁺ T-cells were assessed in ROS^int/low and ROS^high patients using FACS. (B) The mean MFI of the CD3ζ-chain on CD8⁺ T-cells as measured by FACS in ROS^int/low and ROS^high patients. (C) The %al MFI-increase of CD69, HLA-DR, CD137, and CD3ζ-chain on (in presence of CLL cells) anti-CD2, anti-CD3, and anti-CD28 bead-activated CD4⁺ T-cells on addition of 2 mM NAC (n = 12) and the increase of IFNγ release (n = 7) as measured by FACS and ELISA, respectively, after 96 hours of culture. The %al MFI-increase of CD69 as assessed by FACS also after 24 hours (n = 5). (D) A representative FACS analysis of NK-cell subsets (left panel). The mean proportion of the NK-cell subpopulations in ROS^int/low and ROS^high patients is shown as a column diagram (right panel). (E) The MFI of DNAM1, NKG2D, and CD3ζ-chain on NK-cells as measured by FACS in ROS^int/low and ROS^high patients. Bars indicate the standard error mean. Abbreviations: P, P-value; *P < .05; **P < .005. CM, central memory; EM, effector memory; EMRA, terminally differentiated effector memory cells; NA, naïve.