Development of severe anemia in Cbfb-CKO mice. (A) WBC and RBC counts, HGB levels, and platelet counts in the peripheral blood from Vav1-iCre⁺Cbfb^F/+ (Het) and Vav1-iCre⁺Cbfb^F/F (CKO) mice at 6 to 8 weeks (7w) and 12 to 16 weeks (14w) of age. (B) Kaplan-Meier plot showing survival of Vav1-iCre⁺Cbfb^F/+ and Vav1-iCre⁺Cbfb^F/F mice. (C) Ter119 and CD11b expression in total BM cells and splenocytes from Vav1-iCre⁺Cbfb^F/+ and Vav1-iCre⁺Cbfb^F/F mice at 6 to 8 and 12 to 16 weeks of age. Percentages of Ter119⁺ and CD11b⁺ cells are shown. Data are representative of 3 or more biological replicates. Statistical differences were assessed by unpaired Student t test (A) and the log-rank test (B). *P < .05; **P < .01. HGB, hemoglobin.