αIIbβ3 co-localization with talin and kindlin-3 in ADAP^+/+ and ADAP^−/− mouse platelets. ADAP^+/+ and ADAP^−/− mouse platelets stimulated with AYPGKF and processed as in Figure 2 were stained for αIIbβ3 and talin (A-C) or αIIbβ3 and kindlin-3 (D), detected by Alexa-conjugated anti-mouse and anti-rabbit secondary antibodies, respectively. Eight 0.1-μm confocal optical slices were imaged starting at the coverslip and a reconstructed 3-dimensional opacity image was obtained using Volocity software. No signal was seen when IgG control primary antibodies were used. (B-C) Quantification of αIIbβ3 and talin co-localization. (B) Average Pearson correlation coefficient. (C) Quantification of the red/green voxel ratio (n = 8; *P < .05, **P < .02). Results in (B) and (C) represent means ± SEM.