Figure 5
The mut-Stat3 mice have a heightened inflammatory response after infection with C rodentium. Relative expression of IL-12 p40 subunit mRNA in the colons of WT and mut-Stat3 mice 4 days after infection with C rodentium (A). Histology analysis of inflammation present in the distal colon was determined at days 4, 9, and 14 postoral infection with 5 × 109 CFU C rodentium (B). IFN-γ and IL-13 expression in the colonic lamina propria CD4+ T cells at day 14 postinfection was determined by intracellular staining (C). Histograms represent mean values + standard error of the mean, data are representative of 2 independent experiments (n = 8 per group). ns = not significant. *P < .05; **P < .01; ***P < .001.
