White blood cell counts and differential in Srf WT and KO mice and efficiency of Srf deletion. (A) WBC and white cell differential in primary Vav-Cre/Srf WT and KO mice. PB from Vav-Cre/Srf WT and KO mice aged 3-12 days was obtained and WBC and WBC differential assessed on Hemavet. (B) qRT-PCR on neutrophils flow sorted based on Gr-1^high 7/4^high granularity^high criteria from Vav-Cre/Srf WT and KO mice. Srf expression is successfully abrogated in Vav-Cre/Srf KO neutrophils (100-fold, P < .0005; n = 4). *P < .05, **P < .005, ***P < .0005.