Integrin localization in Srf WT and KO neutrophils.Srf WT (left) and KO (right) neutrophils were (A) allowed to adhere to glass coverslips, stained with CD11b fluorescein isothiocyanate, and then stimulated with fMLP for 0 (none) and 15 (fMLP) minutes and stained with wheat germ agglutinin antibody as a membrane stain (without permeabilization) and DAPI (A). (B-C) Srf WT and KO neutrophils were allowed to adhere to glass coverslips, stimulated with fMLP for 0 (none) and 15 (fMLP) minutes and stained with antibodies against CD11b and Clathrin (B) and CD11b and Kindlin (C). (A-B) The top 3 panels show single sections of the Z-stack; the bottom panel shows a merge of the Z-stack. (C) Three sequential Z-stack sections for each image. DAPI stains nuclei. Scale bar = 6 µm; >, leading edge; *_, trailing edge.