Effect of anti-vimentin antibody on flow-dependent platelet adhesion to A1A2A3 protein. Whole blood from human or mice expressing human (h)GPIbα in platelets was mixed with sheep IgG molecule (negative control) or sheep anti-vim antibody (100 μg/mL) and perfused over a surface coated with WT A1A2A3 protein at a shear rate of 1500 seconds−1. After a 2-minute perfusion, the plates were washed with buffer, and attached platelets were recorded and quantified as described in “Methods.” (A) A representative photomicrograph of the effect of the anti-vim antibody on platelet adhesion to the A1A2A3 surface using human blood. (B) The bar graph shows the percent of platelets (mean ± SD) on the surface of 15 different fields of view for human, *P < .03, or mice, **P < .05.
