ITI of multipotent stem cells results in efficient T-cell generation in sublethally irradiated syngeneic recipients. (A) Sublethally irradiated (700 cGy) C57BL/6 recipients received 6000 C57BL/6.CD45.1⁺ LSK cells via ITI 2 to 3 hours after irradiation. Thymuses were harvested on day 60 after radiation and analyzed for host and LSK donor origin. Mean and standard error of the mean (SEM) are presented (n = 4). (B) Animals were treated as described in panel A. Thymuses were harvested on day 60 after radiation and analyzed for CD4 and CD8 DP and SP populations of host and LSK donor origin. Representative plots of 1 of 4 samples are presented. (C) Animals were treated as described in panel A. Thymuses were harvested on day 60 after radiation and analyzed for CD4 and CD8 DN subsets of host and LSK donor origin. Representative plots of 1 of 4 samples are presented. (D) Animals were treated as described in panel A. Spleens were harvested on day 60 after radiation and analyzed for host and donor origin of CD4⁺ and CD8⁺ T cells. Mean and SEM are presented (n = 4). (E) Animals were treated as described in panel A. Spleens were harvested on day 60 after radiation. (i) Splenocytes were analyzed for donor and host origin. (ii) Percentage of T cells in host and donor splenocytes. (iii) Percentage of CD4⁺ and CD8⁺ cells in donor T cells. Representative histograms/plots of 1 of 4 samples are presented.