Figure 5
T-cell proliferation in response to exosomal-protein antigen. C57BL/6 or CD169−/− mice were immunized IV or SC in the forelimb with (A) PBS, 50 µg sucrose cushion purified exosomes derived from B cells cultured with 200 µg/mL ovalbumin protein for 2 days (Exo-pro), and 105 DC-pro or (B) 105 parental B cell-pro. DC and B cells were cultured with 200 µg/mL ovalbumin protein for 2 days. T-cell proliferation of adoptively cotransferred OT-I (CD8) and OT-II (CD4) cells (CFSE or CPD V450) were analyzed 5 days after immunization by flow cytometry. Black line, test group; shaded peak, PBS-immunized mice. Results representative of ≥6 mice per group.
