Hypergammaglobulinemia in Bim^−/−Bmf^−/−mice is associated with increased levels of λ light chain-containing antibodies. (A) Sera were collected from 8- to 10-week-old mice of the indicated genotypes and total Ig, Igλ, and Igκ titers were quantified by ELISA. Overall quantities and λ/κ ratio are depicted based on OD₄₅₀ values. Data from OD values are represented as box plots (n = 5-6 animals per genotype). Box length equals interquartile range. Circles represent minimal and maximal values. Significant differences (P < .05) are indicated *between WT and Bmf^−/−, **between WT and Bim^−/−, ^#between WT and Bim^−/−Bmf^−/−, ^§between Bim^−/− and Bim^−/−Bmf^−/−, and ^&between WT and Bim^−/−Puma^−/− mice (n = 4). (B) Autoantibodies to dsDNA were quantified by ELISA using calf thymus dsDNA for coating of plates and serum samples from 8- to 10-week-old mice of the indicated genotypes. (C) Immunofluorescence staining of paraffin-embedded kidney sections reveals the presence of IgG deposits in glomeruli of female morbid tumor-free Bim^−/−, DKO, and Vav-Bcl-2 transgenic mice (age range, 8-14 months). Healthy Bmf^−/− mice, 18 months of age, were included as control. Magnification, ×400.