Chemokinesis, chemotaxis, and chemotactic index (accuracy) in neutrophils from young and old subjects toward CXCL8 in the presence of PI3K isoform selective inhibitors. Neutrophils from healthy subjects (aged <35 or >65 years of age) (n = 10 each group) migrated toward CXCL8 (100 nM) following incubation with carrier control or the denoted PI3K isoform selective inhibitor for 45 minutes. Measurements were taken from 10 randomly selected cells from each individual: from young donors (A,C,E) and from old donors (B,D,F). The average results for each subject were calculated, and an overall average was used for comparisons between groups. Bars represent the mean migratory parameter with SD shown as the error line (A-F). * indicates significant difference in migratory parameter from carrier control data (P < .05).