Secondary mediators TxA₂ and ADP are key for bacteria-induced platelet aggregation. PRP was incubated for 2 minutes with COX inhibitor indomethacin (10 μM), ADP-receptor P2Y₁₂ inhibitor cangrelor (1 μM), or vehicle (DMSO) prior to addition of bacteria or cross-linked mAb IV.3, and platelet aggregation was monitored. Results are shown as mean ± SD of 3 independent experiments; *P < .05 (see also supplemental Figure 3). DMSO, dimethylsulfoxide.