S aureus Newman induces aggregation of FcγRIIA-transgenic mouse platelets. (A) Comparison of FcγRIIA-transgenic vs wild-type mouse platelets in response to S aureus Newman. Aggregation reactions were performed in mouse PRP. Platelets were stimulated with S aureus Newman plus hIgGs (0.5 mg/mL), with S aureus Newman alone or with hIgG alone. One representative experiment of 3 is shown. (B) Effect of αIIbβ3 inhibitor lotrafiban and Src inhibitor dasatinib on S aureus Newman-induced aggregation of FcγRIIA-transgenic platelets. Aggregation reactions were performed as in panel A in the presence of 0.5 mg/mL hIgG after platelet preincubation with lotrafiban (10 μM), dasatinib (20 μM), or vehicle (DMSO). Results are shown as mean ± SD of 3 independent experiments; *P < .05.