Hematopoietic lineage-specific expression and comparative expression profiles of epigenetic factors in normal hematopoietic cells, progenitor cells, and leukemic cell lines. (A) Heatmap and hierarchical clustering of genes differentially expressed between myeloid and lymphoid lineages (log10-transformed TPM values). (B) Box plots displaying expression levels (TPM values) of the genes in myeloid (M) and lymphoid (L) lineages. P values were calculated with the Mann-Whitney U test (2 sided). (C) PCA analysis of all progenitor samples (red dots), myeloid cells (black dots), lymphoid cells (purple dots), mast and dendritic cells (green dots), and leukemic cell lines (blue triangles) (supplemental Table 1) based on expression of epigenetic factors (TPM values). (D) Bar charts displaying the average transcript levels (TPM values) of SMARCA4, CBX8, CHD2, and EPC1 in normal mature blood cells (gray bars, average of replicates for each cell type), progenitor replicates (blue bars), and leukemic cell lines (orange bars). For cell lines, single datasets were used. (E) Box plots showing the CAGE expression profiles (TPM values) for DNMT1, MLL5, and PRDM2 in leukemic cell lines compared with normal mature hematopoietic cell types and progenitor cells. P values were calculated using the Mann-Whitney U test (2 sided).
