Hematopoietic cells show differential preference for alternative TSS use for selected epigenetic factors. (A-D) Graphs displaying the average percent of expression (TPM) from alternative TSS for RBBP7, HDAC5, CHRAC1, and MLL5 in selected cell types. Percentage is based on sum of TSSs >10 TPM in ≥1 cell type. CAGE tag clusters for alternative TSSs for RBBP7 and HDAC5 are mapped on the sense strand, whereas CHRAC1 and MLL5 are mapped to antisense strand TSS1, 2, and 3 (when applicable) and are shown in gray, orange, and black lines, respectively. Error bars shows the minimum and maximum percent expression values for respective TSS and cell type. The coordinates of the gene location and the TSSs are shown on top of each graph (not to scale). Average TPM values for TSSs in each hematopoietic cell type are shown at the bottom of each graph. (E) Example snap shot from ZENBU genome browser showing expression (TPM) of RBBP7 in B cells, CD8⁺ T cells, and DC monocytes. Expansion of the region surrounding the TSSs in A displays the 2 separate CAGE tag clusters marked in yellow (TSS1) and gray (TSS2).