HeliScopeCAGE-based digital expression analysis. For digital gene expression analysis, tag counts were collected within −500 to +200 bp of GencodeV10 annotated coding genes as outlined in “Materials and methods.” (A) The multidimensional scaling (MDS) plot for replicate HeliScopeCAGE-based digital expression data shows that samples are separated by at least 1 dimension. (B) Numbers of differentially expressed genes in pairwise comparisons of subset samples are given. (C) Two-dimensional hierarchical clustering of 2600 differentially expressed coding genes (normalized to the geometric mean, log2 transformed). C, classical; I, intermediate; N, nonclassical monocyte subset. (D) Digital gene expression data for the top 20 monocyte (MO) subset-specific genes.