Different helminth infections induce either monocyte-derived or tissue-derived AAMs. (A) Representative FACs plots of pleural cavity macrophages on day 12 after L sigmodontis infection compared with pleural cavity macrophages of mice untreated (Res) or injected i.p. with thioglycollate alone (Thio) or Thio and IL-4c (Thio+IL-4c) or IL-4c alone (IL-4c). (B) Graphs depict proportion of CD206 and PD-L2 positive pleural cavity macrophages. (C) Representative FACs plots of PD-L2 and CD206 expression gated on CD11b⁺ cells isolated from the liver of S mansoni-infected mice (8 weeks after infection) compared with CD11b⁺ cells isolated from the liver of mice untreated (naïve) mice. (D) FIZZ1/RELMa intracellular staining on PD-L2⁺CD206⁺ macrophages from S mansoni-infected livers compared with PD-L2⁻CD206⁻ macrophages. (E) Graph depicts the percentage of PD-L2 and CD206 double positive cells, gated on CD11b⁺ cells, from the livers of individual mice. (F) MFI of FIZZ1/RELMa staining on PD-L2⁺CD206⁺ and PD-L2⁻CD206⁻ macrophages from livers of individual infected mice. *P < .05 and **P < .01 as determined by ANOVA. Results shown representative of 2 independent experiments.